to set up the software for your use. This guide shows a workflow that uses application settings. BD FACSDiva Software Quick Reference Guide for the BD LSR II. Compensation Controls in the BD FACSDiva Software Reference Manual. • Default templates are now provided for certain instrument functions. If you are the first user of the day: Boot up the computer and log on to Windows. Wait 10 seconds, then Open the BD FACSDiva software and log on. Allow the.

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No part of this publication may be reproduced, transmitted, transcribed, stored in retrieval systems, or translated into any language or computer language, in any form or by any means: The information in this guide is subject to change without notice. BD Biosciences reserves the right to change its products and services at any time to incorporate the softwaare technological developments.

Msnual this guide has been prepared with every precaution to ensure accuracy, BD Biosciences assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information.

BD Biosciences welcomes customer input on corrections and suggestions for improvement.

This software is the property of Becton, Dickinson and Company. Each sale of a stored unit of this software grants the purchaser a nontransferable, nonexclusive, personal license. This software may not be duplicated, teference, or copied in any form or by any means whatsoever, except as otherwise permitted by law.

Cy dyes are subject to proprietary rights of Amersham Biosciences Corp. Any other use requires a commercial sublicense from Amersham Biosciences Corp. Microsoft and Windows are registered trademarks of Microsoft Corporation. All other company and product names may be trademarks of the respective companies with which they are associated. Refer to the information supplied by the manufacturer for application-specific limitations.

See the New Features section for details. If you are a new user, use the tutorials to get started with the software. If you are an experienced user, try the updated tutorials to become softwre with new software features. Updated features are highlighted in the New Features section. New Cytometer Setup and Tracking features work in conjunction with a new bead reagent to provide: A complete baseline characterization of the cytometer Automated performance checking and tracking of characterization measurements A new feature to maintain application-specific settings For a brief overview of Cytometer Setup and Tracking, see Previewing Cytometer Setup and Tracking Features on page See Creating a New User on page 21 in this guide.

New custom fields in the Account Administration dialog allow the administrator to reterence a name and value for each user profile. For example, a custom name field could be Account Number with the value field displaying a numeric value.


BD FACSDiva Files

Administrators can access the latest user tracking log file through the File menu. Also, new columns of facsdiga have been added including software build version, cytometer, serial number, and the custom manuall described above. All configuration management tasks such as set current, create, edit, and delete are done through the new Cytometer Setup and Tracking feature.

See Publishing and Presenting Data on page 73 in this guide. New scaling selections are available for linear and channel values to support applications. Simpler Gating Features Proportional resizing of polygon and rectangular gates. See Editing Gates on page 48 in this guide. Drag and drop gates within a plot or from one plot to another within a rreference. Drag and drop gates in the population hierarchy. New feature to apply gate xoftware such as shape and size between worksheets to other tubes in an open experiment.

About This Guide 7. Easily create new labels from the pre-defined Labels List. Copy and paste rows and columns. Set stopping time and stopping and storage gates for specific tubes or wells. Assign preferred global worksheets.

Rename tubes, wells, and specimens. Events to Record field maintains the last entered value. Stopping Time selection maintains the last entered value. Display a grid on the worksheet that is scalable.

BD FACSDiva Software Reference Manual

referencs Worksheet toolbar button to save the worksheet as a PDF file. Export selected worksheet elements as an XML file including plots, text boxes, statistics, and population hierarchies. See Publishing and Presenting Data on page 73 of this guide. Carousel Report now includes the directory selected for each specimen s data in the carousel. Create and modify compensation controls in the Plate window.

Rename specimens in the Plate window. Enable plate controls in the Plate window. Copy and paste wells to blank positions in the Plate window. Choose apply and export panel templates in the Plate window. About This Guide 9. Improved indicators in the Tacsdiva to show where sort layouts are being created.

Addition of experiment and specimen information to Sort Sofgware. New default Sort Precision Model: Persistence of the target events count. Create label-specific information for compensation tubes without having to create tubes.

Add a description to keywords in the Inspector window.

User preference to prevent display of data in plots until the recording is completed. Record and save large data files of up to 10 million events. Users who have access can edit setups.

Conventions The following table lists conventions used in this guide. When used with key names, a dash means to press two keys simultaneously.

For example, Ctrl-P means to hold down the Control key while pressing the letter p. Technical Assistance For technical questions or assistance in solving a problem: Locate topics specific to the operation you are performing.

To quickly find information on any topic, enter search terms on the Search tab. You can search individual books, or search all books at once.


You can also use the Contents or Index tab to find information. To find software information, click to expand topics in the Software Help book; to find cytometer information, expand the Cytometer Help book. Refer to the Faczdiva section in the Software or Cytometer Help books. About This Guide When contacting BD Biosciences, have the following information available: For cytometer support from within the US, softwarfprompt 2, 2. After the upgrade, restart your cytometer and computer as described in the upgrade instructions.

No password is required the first time you log into the software. For more information about user login, see Using Administrative Options on page Hide or show windows by clicking buttons on the workspace toolbar 1 in the figure. Some software features are cytometer-specific, so your workspace components might look different from those shown in this example. For information about cytometer-specific components, refer to your cytometer manual.

Getting Started with BD FACSDiva Software

Most windows can be resized sofware dragging a border or corner. Becoming Familiar with the Workspace Only one experiment can be open at a time. In an open experiment, you can add, rename, or copy elements, and record or display data. Click a button on the Browser toolbar a to add elements to the Browser. To display fewer experiments, enter a term in the search field b and click the Find button d. Basic controls are always shown.

Your Dashboard might contain more controls majual those shown depending on your cytometer type and options. When you are connected to a cytometer, the window also shows status messages.

If an experiment is open and the current tube pointer is set, softtware window displays cytometer settings for the current acquisition tube and laser controls. Additional controls might be displayed in this window depending on the cytometer you are running; refer to your cytometer manual for a description. Use the Inspector to view or modify the attributes of one or more objects in the worksheet or Browser.

The contents of the Inspector change depending on what is selected. For example, this Inspector shows plot options. Plots on a global worksheet show data for only one tube at a time, the tube osftware is selected with the current tube pointer. These worksheets mwnual indicated by green tabs a.

Plots on a normal worksheet are tube-specific. These worksheets are indicated by tabs that are white when active and gray when inactive.

Click the Worksheets view button on the Worksheet toolbar b to switch between facsdvia or normal worksheets. Then you can create analysis objects or free text or align objects on a worksheet.