The isolation and regeneration of potato (Solanum tuberosum L) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid . The Isolation of regeneratiom of potato (Solanum tuberosum L.) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid. One of new technologies in order to create hybrid plant from different species is protoplast fusion. This technique involves protoplast isolation, purification.
|Published (Last):||16 October 2018|
|PDF File Size:||13.33 Mb|
|ePub File Size:||11.18 Mb|
|Price:||Free* [*Free Regsitration Required]|
Prosiding Seminar Nasional Biologi
Funding for sustainable Open Access incl. The obyectives of the research was to investigate the effect of combination treatment of sucrose concentrations and incubation time on protoplast isolation of the leaves mesophyll orchid of Paraphalaenopsis layockii.
Protoplast were cultured on the medium of Ikeda and Uchimia. Close Go to Workspace. The results showed that there was effect of treatment for all variables observed.
Journal Homepage Journal Title: Cell wall generation was observed. The highest protoplast density and number of viable protoplasts achieved in the combined treatment of 0.
Isolation of protoplast from soybean, cowpea, and tobacco and their fusion. Reference Number Reference Number. The first factor was concentrations of sucrose which consists of three levels namely 0.
They were incibated in full darkness at 27 Celcius centigrade by constant shaking at 50 rpm orbitor shaker. Isolasi dan fusi protoplas dari kedelai, buncis, dan tembakau. On both medium leaf protoplast, irradiated protoplasts and their fused do not regenerate cell wall and all cultured died out within four weeks incubation.
Standard Search Advanced Search. Protoplast were collected by centrifugation of g and a thin layer of the suspension was irradiated with ultraviolet isolwsi.
Our members Our publisher members Our sponsors Our volunteers. The second factor was incubation time which consists of four levels namely 2 hours, 4 hours, 6 hours and 8 hours.
Isolasi Protoplas Tanaman Kacang Panjang Secara Enzimatis
Indonesia Language of fulltext: Leaf protoplast were releasaed in h, soybean and tobacco callus protoplast in h, and cowpeas callus protoplast in h of incubation. Indonesian, English Full-text formats available: Get permanent URL for this record.
Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney test.
Isolasi Protoplas secara Enzimatis pada Tanaman Kecipir | Riyadi | Buletin Plasma Nutfah
Fusion was induced with PEG solution according to Uchimia and fused protoplasts were collected by centrifugation of g. Regeneration of cell wall observed progessively in mother protoplast from tobacco, cowpea A27, E1, and H6 and fused protoplast of soybean with tobacco, tobacco with cowpea C4, E1, and H6soybean with cowpea C4 and between cowpea C4 and cowpea E1.
For soybean leaf, the medium was enriched with driselase and pectolyase Y Please provide prottoplas name for this query: Callus wich has been two times resubcultured was suspended in prtooplas digestive medium without driselase, CaCl2, and MES and incubated in lowlight intensity by protopas shaking at rpm in reciprocal water shaker at 30 celcius centigrade.